Database : HANSEN Search on : SEQUENCIA DE BASES [Subject descriptor] References found : 52 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 17562 Author: Kang, Tae Jin; Yeum, Chung Eun; Kim, Byoung Chul; You, Eun-Young; Chae, Gue-Tae Title: Differential production of interleukin-10 and interleukin-12 in mononuclear cells from leprosy patients with a Toll-like receptor 2 mutation ..- Source: s.l; s.n; 2004. 7 p. ilus, tab, graf. Abstract: Toll-like receptor 2 (TLR2) is a key mediator of the immune response to mycobacterial infections, and mutations in TLR2 have been shown to confer susceptibility to infection with mycobacteria. This study investigated the profiles of cytokines, such as interferon (IFN)-gamma, interleukin (IL)-10, IL-12 and tumour necrosis factor (TNF)-alpha in response to Mycobacterium leprae in peripheral blood mononuclear cells (PBMC) with the TLR2 mutation Arg677Trp, a recently reported polymorphism that is associated with lepromatous leprosy. In leprosy patients with the TLR2 mutation, production of IL-2, IL-12, IFN-gamma, and TNF-alpha by M. leprae-stimulated PBMC were significantly decreased compared with that in groups with wild-type TLR2. However, the cells from patients with the TLR2 mutation showed significantly increased production of IL-10. There was no significant difference in IL-4 production between the mutant and wild-type during stimulation. Thus, these results suggest that the TLR2 signal pathway plays a critical role in the alteration of cytokine profiles in PBMC from leprosy patients and the TLR2 mutation Arg677Trp provides a mechanism for the poor cellular immune response associated with lepromatous leprosy. (AU). Descriptors: Sequência de Bases Interleucina-10/*BI Interleucina-12/*BI Hanseníase/GE/*IM Leucócitos Mononucleares/IM Glicoproteínas de Membrana/*GE/ME Camundongos Nus Dados de Sequência Molecular Mutação Puntual/* Receptores da Superfície Celular/*GE/ME Receptor 2 Toll-Like Receptores Toll-Like Fator de Necrose Tumoral alfa/BI Limits: Adulto Idoso Animais Feminino Humanos Masculino Camundongos Research Support, Non-U.S. Gov't Meia-Idade Location: BR191.1; 09341/s

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Id: 17284 Author: Moraes, M. O; Sarno, E. N; Almeida, A. S; Saraiva, B. C. C; Nery, J. A. C; Martins, R. C. L; Sampaio, E. P Title: Cytokine mRNA expression in leprosy; a possible role for interferon-gamma and interleukin-12 in reaction (RR and ENL) ..- Source: s.l; s.n; 1999. 9 p. tab, graf, ilus. Abstract: Leprosy patients during the natural course of the disease may develop reactional episodes, namely reversal reaction (RR) and erythema nodosum leprosum (ENL). Immunological events described as occurring during RR indicate up-regulation of the immune response, whereas in ENL the events are not fully understood. The aim of this study was to analyse the in vivo pattern of cytokine gene expression in the reactional states of leprosy. Peripheral blood mononuclear cells (PBMC, n = 14) and tissue samples (n = 17) obtained from patients with ENL and RR were obtained and assayed by RT-PCR. PBMC obtained from unreactional patients (n = 15) and normal individuals (n = 5) were also assessed. Expression of interferon (IFN)gamma, granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin (IL)-2Rp55, perforin and IL-1beta mRNA in PBMC were detected mostly in ENL/RR patients, but not in unreactional patients. Likewise, cytokines such as IL-6, IL-8, tumour necrosis factor (TNF)alpha and TNFbeta were also present in reactional and tuberculoid patients as opposed to lepromatous leprosy (BL/LL). Interestingly, the majority of ENL/RR patients showed messages for IL-6, IL-10, IL-12 and TNFalpha in the skin. IFNgamma was detected in 84.6% (ENL) and 100% (RR) of the patients, whereas IL-4 was detected only in few individuals (38.5 and 25%, respectively). Although mRNA expression and protein levels may be different, the data reported in this study suggest a cytokine mRNA profile that seems to be indistinguishable for RR and ENL. In addition, it shows up-regulation of immuno-inflammatory cytokines in the blood and tissue of the same patient examined before and during reaction. Furthermore, it is suggested that this pattern of response results from an immunological reactivation that might lead to an acute inflammatory response in both reactional episodes. (AU). Descriptors: Sequência de Bases Estudos de Casos e Controles Citocinas/*GE Primers do DNA/GE Expressão Gênica Fator Estimulador de Colônias de Granulócitos-Macrófagos/GE Interferon Tipo II/*GE Interleucina-12/*GE Hanseníase/*GE/*IM Glicoproteínas de Membrana/GE Reação em Cadeia da Polimerase RNA Mensageiro/BL/*GE/*ME Limits: Humano Support, Non-U.S. Gov't Location: BR191.1; 09183/s

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Id: 13619 Author: Kang, Tae Jin; Lee, Seong-Beom; Chae, Gue-Tae Title: A polymorphism in the toll-like receptor 2 is associated with IL-12 production from monocyte in lepromatous leprosy ..- Source: s.l; s.n; Oct. 2002. 7 p. graf. Abstract: Toll-like receptor 2 (TLR2) is critical in the immune response to mycobacterial infections, and the mutations in the TLR2 have been shown to confer the susceptibility to infection with mycobacteria. We previously reported the detection of TLR2 Arg677Trp mutation in lepromatous leprosy. Here, the events triggered by TLR2 in response to cell lysate of Mycobacterium leprae(MLL), the causative agent of leprosy, were investigated. Upon stimulation with MLL, monocytes produced TNF-alpha and Interleukin-12 (IL-12), which play a role in the innate immune response to infection. Anti-TLR2 mAb blocked greater than 50 per cent of the MLL-induced production of IL-12. We also performed the functional study on TLR2 by measurement of IL-12 production in serum and monocytes from leprosy patients with TLR2 mutation (Arg677Trp). The monocytes obtained from patients with the TLR2 mutation, in comparison to the wild-type TLR2, is significantly less responsive to MLL. It was also confirmed that patients with TLR2 mutation showed significantly lower serum levels of IL-12, in comparing with TLR2 wild-type. Our results reveal that innate immune response of monocytes against M. lepraeis mediated by TLR2, and suggest that the mutation in the intracellular domain of TLR2 gene is associated with IL-12 production in lepromatous leprosy. (AU). Descriptors: SEQUÊNCIA DE AMINOACIDOS SEQUÊNCIA DE BASES ESTUDOS DE CASOS E CONTROLES DNA COMPLEMENTAR/genet IMUNIDADE NATURAL INTERLEUCINA-12/bios HANSENIASE VIRCHOWIANA/genet HANSENIASE VIRCHOWIANA/imunol GLICOPROTEINAS DE MEMBRANA/quim GLICOPROTEINAS DE MEMBRANA/genet MONOCITOS/imunol MYCOBACTERIUM LEPRAE/imunol MUTACAO PUNTUAL POLIMORFISMO (GENETICA) ESTRUTURA TERCIARIA DE PROTEINA RECEPTORES DA SUPERFICIE CELULAR/quim RECEPTORES DA SUPERFICIE CELULAR/genet FATOR DE NECROSE TUMORAL/bios Limits: HUMANO SUPPORT, NON-U.S. GOV'T HUMANO IN VITRO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09004/s

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Id: 12309 Author: Kang, Tae-Jin; You, Ji-Chang; Chae, Gue-Tae Title: Identification of catalase-like activity from mucobacterium leprae and the relationship between catalase and isonicotinic acid hydrazide (INH) ..- Source: s.l; s.n; 2001. 7 p. ilus, tab, graf. Descriptors: SULFATO DE AMONIO ANTITUBERCULOSOS SEQUENCIA DE BASES BENZIDINAS CATALASE CATALASE PRIMERS DO DNA DNA COMPLEMENTAR ELETROFORESE EM GEL DE AGAR PEROXIDO DE HIDROGENIO ISONIAZIDA HANSENIASE HANSENIASE MACROFAGOS PERITONEAIS CAMUNDONGOS CAMUNDONGOS ENDOGAMICOS BALB C MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE - PEROXIDASE  PEROXIDASES  PEROXIDASES  REAÇAO EM CADEIA POR POLIMERASE  DNA POLIMERASE DIRIGIDO POR RNA  CONTAGEM DE CINTILAÇAO  HOMOLOGIA DE SEQUENCIA DO ACIDO NUCLÉICO  ESPECTROFOTOMETRIA Location: BR191.1; 08638/s

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Id: 12292 Author: Ohara, Naoya; Matsuoka, Masanori; Nomaguchi, Hiroko; Naito, Mariko; Yamada, Takeshi Title: Protective responses against experimental mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates ..- Source: s.l; s.n; Feb. 2001. 5 p. ilus, graf. Descriptors: ANTIGENOS DE BACTÉRIAS VACINA BCG VACINA BCG VACINA BCG SEQUENCIA DE BASES PRIMERS DO DNA HANSENIASE HANSENIASE CAMUNDONGOS CAMUNDONGOS ENDOGAMICOS BALB C CAMUNDONGOS ENDOGAMICOS C57BL MYCOBACTERIUM BOVIS MYCOBACTERIUM BOVIS Limits: ANIMAL Location: BR191.1; 08623/s

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Id: 12248 Author: Williams, Diana L; Pittman, Tana L; Gillis, Thomas P; Matsuoka, Masanori; Kashiwabara, Yoshiko Title: Simultaneous detection of mycobacterium leprae and its susceptibility to dapsone using DNA heteroduplex analysis ..- Source: s.l; s.n; Jun. 2001. 6 p. ilus, tab. Descriptors: SEQUENCIA DE BASES DNA BACTERIANO DNA BACTERIANO DAPSONA RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE SENSIBILIDADE E ESPECIFICIDADE ANALISE DE SEQUENCIA DE DNA Análise Heteroduplex/*MT Location: BR191.1; 08678/s; BR191.1; 08430/s

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Id: 12238 Author: Maeda, Shinji; Matsuoka, Masanori; Nakata, Noboru; Kai, Masanori; Maeda, Yumi; Hashimoto, Ken; Kimura, Hiroaki; Kobayashi, Kazuo; Kashiwabara, Yoshiko Title: Multidrug resistant mycobacterium leprae from patients with leprosy ..- Source: s.l; s.n; Dec. 2001. 5 p. tab, graf. Descriptors: AGENTES ANTIINFECCIOSOS DE FLUOROQUINOLONA AGENTES ANTIINFECCIOSOS DE FLUOROQUINOLONA ANTIBIOTICOS ANTITUBERCULOSE ANTIBIOTICOS ANTITUBERCULOSE SEQUENCIA DE BASES PRIMERS DO DNA DNA BACTERIANO DNA BACTERIANO RESISTENCIA MICROBIANA A DROGAS RESISTENCIA A MULTIPLAS DROGAS GENES BACTERIANOS GENES BACTERIANOS LEPROSTATICOS LEPROSTATICOS HANSENIASE HANSENIASE CAMUNDONGOS DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE OFLOXACINA OFLOXACINA RIFAMPINA RIFAMPINA REACAO EM CADEIA POR POLIMERASE VIA TRANSCRIPTASE REVERSA Limits: ANIMAL Location: BR191.1; 08640/s

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Id: 11944 Author: Visca, Paolo; Fabozzi, Giulia; Petrucca, Andrea; Ciaccio, Chiara; Coletta, Massimo; De Sanctis, Giampiero; Bolognesi, Martino; Milani, Mario; Ascenzi, Paolo Title: The truncated hemoglobin from Mycobacterium leprae ..- Source: s.l; s.n; 2002. 7 p. ilus, graf. Descriptors: MYCOBACTERIUM LEPRAE SEQUENCIA DE AMINOACIDOS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS SEQUENCIA DE BASES CLONAGEM MOLECULAR HEMOGLOBINAS HEMOGLOBINAS HEMOGLOBINAS LIGANTES MODELOS MOLECULARES DADOS DE SEQUENCIA MOLECULAR PROTEINAS RECOMBINANTES PROTEINAS RECOMBINANTES HOMOLOGIA DE SEQUENCIA DE AMINOACIDOS Location: BR191.1; 08601/s

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Id: 11729 Author: Nishimura, Masayuki; Kwon, Kyung Sool; Shibuta, Kenji; Yoshikawa, Yasuji; Oh, Chang-Keun; Suzuki, Tomokazu; Chung, Tae-Ahn; Hori, Yoshiaki Title: An improved method for DNA diagnosis of leprosy using formaldehyde-fixed, paraffin-embedded skin biopsies ..- Source: s.l; s.n; 1994. 4 p. ilus, tab. Descriptors: HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE SEQUENCIA DE BASES DNA BACTERIANO ELETROFORESE EM GEL DE AGAR FORMALDEIDO DADOS DE SEQUENCIA MOLECULAR INCLUSAO EM PARAFINA REAÇAO EM CADEIA POR POLIMERASE SEQUENCIAS REGULADORAS DE ACIDO NUCLÉICO SENSIBILIDADE E ESPECIFICIDADE PELE FIXAÇAO DE TECIDOS Location: BR191.1; 07218/s

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Id: 11686 Author: Ohara, Naoya; Matsuoka, Masanori; Nomaguchi, Hiroko; Naito, Mariko; Yamada, Takeshi Title: Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates ..- Source: s.l; s.n; 2001. 5 p. ilus, graf. Descriptors: MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM BOVIS MYCOBACTERIUM BOVIS HANSENIASE HANSENIASE ANTIGENOS DE BACTÉRIAS VACINA BCG VACINA BCG VACINA BCG SEQUENCIA DE BASES PRIMERS DO DNA CAMUNDONGOS ENDOGAMICOS BALB C CAMUNDONGOS ENDOGAMICOS C57BL PLASMIDEOS BAÇO Limits: ANIMAL MASCULINO CAMUNDONGOS Location: BR191.1; 08478/s

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Id: 11681 Author: Rambukkana, Anura Title: M. leprae genome sequence ..- Source: s.l; s.n; 2001. 1 p. . Descriptors: HANSENIASE MYCOBACTERIUM LEPRAE SEQUENCIA DE BASES GENOMA BACTERIANO ANALISE DE SEQUENCIA DE DNA Location: BR191.1; 08473/s

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Id: 11607 Author: Williams, Diana L; Pittman, Tana L; Gillis, Thomas P; Matsuoka, Masanori; Kashiwabara, Yoshiko Title: Simultaneous detection of mycobacterium leprae and its susceptibility to dapsone using DNA heteroduplex analysis ..- Source: s.l; s.n; 2001. 6 p. tab, graf. Descriptors: SEQUENCIA DE BASES DNA BACTERIANO DNA BACTERIANO DAPSONA RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE SENSIBILIDADE E ESPECIFICIDADE ANALISE DE SEQUENCIA DE DNA ANALISE HETERODUPLEX/*MT Location: BR191.1; 08430/s

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Id: 11594 Author: Martin, E; Roche, P. W; Triccas, J. A; Britton, W. J Title: DNA encoding a single mycobacterial antigen protects against leprosy infection ..- Source: s.l; s.n; 2001. 6 p. tab. Descriptors: ANTIGENOS DE BACTÉRIAS VACINA BCG VACINAS BACTERIANAS VACINAS BACTERIANAS VACINAS BACTERIANAS SEQUENCIA DE BASES PRIMERS DO DNA DNA BACTERIANO DNA BACTERIANO INTERFERON TIPO II HANSENIASE HANSENIASE HANSENIASE CAMUNDONGOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE LINFOCITOS T VACINAS DE DNA/GE/IM/*PD Limits: ANIMAL Location: BR191.1; 08417/s

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Id: 11316 Author: Shimoji, Yoshihiro; Ng, Vincent; Matsumura, Kiichiro; Fischetti, Vincent A; Rambukkana, Anura Title: A 21-kDa surface protein of mycobacterium leprae binds peripheral nerve laminin-2 and mediates Schwann cell invasion ..- Source: s.l; s.n; 1999. 6 p. tab, graf. Descriptors: SEQUENCIA DE AMINOACIDOS ADESINAS BACTERIANAS ADESINAS BACTERIANAS SEQUENCIA DE BASES ELETROFORESE EM GEL DE POLIACRILAMIDA LAMININA HANSENIASE CAMUNDONGOS CAMUNDONGOS ENDOGAMICOS BALB C MICROSCOPIA IMUNOELETRONICA DADOS DE SEQUENCIA MOLECULAR PESO MOLECULAR MYCOBACTERIUM LEPRAE SISTEMA NERVOSO PERIFÉRICO SISTEMA NERVOSO PERIFÉRICO CÉLULAS DE SCHWANN PROPRIEDADES DE SUPERFICIE CELULAS CULTIVADAS Limits: ANIMAL Location: BR191.1; 07255/s

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Id: 11299 Author: Moraes, M. O; Sarno, E. N; Almeida, A. S; Saraiva, B. C. C; Nery, J. A. C; Martins, R. C. L; Sampaio, E. P Title: Cytokine mRNA expression in leprosy: a possible role for interferon-gamma and interleukin-12 in reactions (RR and ENL) ..- Source: s.l; s.n; 1999. 9 p. ilus, tab, graf. Descriptors: SEQUENCIA DE AMINOACIDOS SEQUENCIA DE BASES ESTUDOS DE CASOS E CONTROLES CITOCINAS PRIMERS DO DNA EXPRESSAO GENICA FATOR ESTIMULADOR DE COLONIAS DE GRANULOCITOS-MACROFAGOS INTERFERON TIPO II INTERLEUCINA-12 HANSENIASE HANSENIASE GLICOPROTEINAS DE MEMBRANAS REAÇAO EM CADEIA POR POLIMERASE RNA MENSAGEIRO RNA MENSAGEIRO RNA MENSAGEIRO Location: BR191.1; 07238/s

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Id: 11147 Author: Spigelman, Mark; Donoghue, Helen D Title: Brief communication: unusual pathological condition in the lower extremities of a skeleton from ancient Israel ..- Source: s.l; s.n; 2001. 2 p. tab. Descriptors: HANSENIASE HANSENIASE ANTROPOLOGIA FISICA SEQUENCIA DE BASES DNA DIAGNOSTICO DIFERENCIAL FOSSEIS MADUROMICOSE DADOS DE SEQUENCIA MOLECULAR REAÇAO EM CADEIA POR POLIMERASE Location: BR191.1; 07318/s

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Id: 11016 Author: Triccas, James A; Winter, Nathalie; Roche, Paul W; Gilpin, Andrea; Kendrick, Kathleen E; Britton, Warwick J Title: Molecular and immunological analyses of the mycobacterium avium homolog of the immunodominant mycobacterium leprae 35-kilodalton protein ..- Source: s.l; s.n; 1998. 7 p. ilus, tab, graf. Descriptors: HANSENIASE MYCOBACTERIUM AVIUM MYCOBACTERIUM AVIUM MYCOBACTERIUM LEPRAE SEQUENCIA DE AMINOACIDOS SEQUENCIA DE BASES CLONAGEM MOLECULAR GENES BACTERIANOS COBAIAS PROTEINAS DO CHOQUE TÉRMICO PROTEINAS DO CHOQUE TÉRMICO HIPERSENSIBILIDADE TARDIA EPITOPOS IMUNODOMINANTES EPITOPOS IMUNODOMINANTES LEUCOCITOS MONONUCLEARES TRANSFORMAÇAO LINFOCITICA DADOS DE SEQUENCIA MOLECULAR PROTEINAS RECOMBINANTES HOMOLOGIA DE SEQUENCIA DE AMINOACIDOS TUBERCULOSE PULMONAR Location: BR191.1; 07286/s

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Id: 10325 Author: Rafi, Abdolnasser; Feval, Francois Title: PCR to detect mycobacterium tuberculosis DNA in sputum samples from treated leprosy patients with putative tuberculosis ..- Source: s.l; s.n; 1995. 5 p. tab. Descriptors: FATORES ETARIOS SEQUENCIA DE BASES ESTUDOS DE CASOS E CONTROLES DNA BACTERIANO INCIDENCIA IRA (GEOGRAFICO) HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM TUBERCULOSIS MYCOBACTERIUM TUBERCULOSIS REAÇAO EM CADEIA POR POLIMERASE SENSIBILIDADE E ESPECIFICIDADE ESCARRO TESTE TUBERCULINICO TUBERCULOSE TUBERCULOSE TUBERCULOSE Location: BR191.1; 07175/s

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Id: 10315 Author: Haas, Christian J; Zink, Albert; Palfi, Gyorgy; Szeimies, Ulrike; Nerlich, Andreas G Title: Detection of leprosy in ancient human skeletal remains by molecular identification of Mycobacterium leprae ..- Source: s.l; s.n; 2000. 9 p. ilus, graf. Descriptors: SEQUENCIA DE BASES OSSO E OSSOS DNA BACTERIANO HISTORIA DA MEDICINA MEDIEVAL HISTORIA DA MEDICINA MODERNA HANSENIASE HANSENIASE HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE PALEONTOLOGIA PEPTIDIOS CICLICOS REAÇAO EM CADEIA POR POLIMERASE ANALISE DE SEQUENCIA DE DNA Location: BR191.1; 07384/s

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Id: 10299 Author: Roy, S; Frodsham, A; Saha, B; Hazra, K. S; Maccie-Taylor, C. G. N; Hill, A. V. S Title: Association of vitamin D receptor genotype with leprosy type ..- Source: s.l; s.n; 1999. 5 p. tab. Descriptors: ALELOS SEQUENCIA DE BASES PROTEINAS PRIMITIVAS DE ADENOVIRUS PROTEINAS DE TRANSPORTE ESTUDOS DE CASOS E CONTROLES PRIMERS DO DNA FREQUENCIA DOS GENES GENOTIPO IMUNOGENÉTICA INDIA HANSENIASE HANSENIASE HANSENIASE LEPROMATOSA HANSENIASE LEPROMATOSA HANSENIASE TUBERCULOIDE HANSENIASE TUBERCULOIDE PROTEINAS DA MEMBRANA POLIMORFISMO (GENÉTICA) RECEPTORES DE CALCITRIOL RECEPTORES DE CALCITRIOL DELEÇAO DE SEQUENCIA VARIAÇAO (GENÉTICA) Location: BR191.1; 07402/s

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